Isolation of genes negatively or positively co-expressed with human recombination activating gene 1 (RAG1) by differential display PCR (DD RT-PCR)
نویسندگان
چکیده
منابع مشابه
Isolation of genes negatively or positively co-expressed with human recombination activating gene 1 (RAG1) by differential display PCR (DD RT-PCR).
Differential display PCR (DD RT-PCR) has been extensively used for analysis of differential gene expression, but continues to be hampered by technical limitations that impair its effectiveness. In order to isolate novel genes co-expressing with human RAG1, we have developed an effective, multi-tiered screening/purification approach which effectively complements the standard DD RT-PCR methodolog...
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~The first two authors contributed equally to the establishment of the method and to the results obtained. 4present address: Max-Planck-Gesellschaft, MaxDelbr~ick-Haus, 13122 Berlin-Buch, Germany. Analysis of gene expression is a central aim in most studies in molecular and cell biology. Interest in tissue-specific gene expression and, in particular, in changes in the expression patterns occurr...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.19.4497